Product Advantages

• Animal component-free; Contains only recombinant proteins and synthetic components
• Greater single-cell cloning efficiency of CHO cells compared with chemically defined, protein-free media, particularly at low plating densities

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ClonaCell™-CHO ACF is a methylcellulose-based semi-solid medium with supplements for the selection and cloning of suspension-adapted CHO cells in serum-free conditions. This medium is animal component-free and contains only recombinant proteins and synthetic components. Single-cell cloning efficiency of CHO cells is higher in this medium than in protein-free media, especially at low plating densities. It does not contain L-glutamine, selection agents, or phenol red and is compatible with dihydrofolate reductase (DHFR) and glutamine synthase (GS) selection systems. Benefits of semi-solid cloning: Individual cells are suspended in viscous medium and form physically separated, discrete colonies that are easily isolated. Monoclonal cell lines are isolated in less time using fewer resources compared with selection and cloning by limiting dilution. Diverse clones with a wide range of growth rates and productivities form discrete colonies in the viscous medium. As a result, rare and high-producing clones can be individually isolated more easily using simultaneous selection and cloning in semi-solid medium compared with selection in bulk liquid cultures. CONTAINS Methylcellulose Recombinant proteins (animal component-free) Other ingredients SUBTYPE 专业培养基, 半固体培养基 CELL TYPE CHO细胞 SPECIES 其他 APPLICATION 半固体克隆, 细胞培养 BRAND ClonaCell AREA OF INTEREST 抗体制备, 细胞系制备, 药物发现和毒性测试 FORMULATION 无动物源成分, 无血清