Product Advantages

• Enables single-cell cloning and expansion of CHO cells in a chemically defined, protein-free, animal component-free medium

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ClonaCell™-CHO CD Medium is a methylcellulose-based semi-solid medium recommended for selection and cloning of Chinese hamster ovary (CHO) cells. This medium is chemically defined, protein-free, and animal component-free. It does not contain L-glutamine, selection agents, or phenol red and is compatible with dihydrofolate reductase (DHFR) and glutamine synthetase (GS) selection systems. Benefits of semi-solid cloning: Individual clones and their progeny remain localized together in a semi-solid matrix as they grow to form distinct monoclonal colonies. This prevents the loss of rare, high-producing clones by overgrowth from faster-growing cells, as can occur during selection in a liquid medium, and facilitates the isolation of a diverse set of clones with a wide range of growth rates and productivities to be obtained for downstream screening. Colonies obtained from semi-solid medium have a high probability of monoclonality, allowing clonal cell lines to be generated in less time and using fewer resources than with limiting dilution cloning. Colonies can be easily picked from the semi-solid medium by manual or robotic methods and dispersed into a liquid growth medium for screening and expansion. CONTAINS Methylcellulose Kolliphor® P188 Other ingredients SUBTYPE 专业培养基, 半固体培养基 CELL TYPE CHO细胞 SPECIES 其他 APPLICATION 半固体克隆, 细胞培养 BRAND ClonaCell AREA OF INTEREST 抗体制备, 细胞系制备, 药物发现和毒性测试 FORMULATION 无动物源成分, 无血清, 化学成分确定, 无蛋白质