ddPCR is a form of PCR that provides absolute quantification, compared to the relative quantification of qPCR and semi-quantification of traditional PCR. It is based on a partitioning principle: the sample containing target DNA molecules is divided into 20,000 nanoliter-sized droplets. Each droplet represents an individual PCR reaction that occurs during the amplification process.

Advantages of ddPCR over other forms of PCR are its higher sensitivity, precision, and accuracy. It is practical and easily scalable and delivers reliable, reproducible results. Unlike with qPCR, there is no need to use standard curves on any references in calculating the absolute concentrations of the target template.

ddPCR has taken its rightful place in clinical settings for allele detection, rare mutation detection, determination of copy number variation, gene expression analysis, and more. It is instrumental in pinpointing rare sequences in a wild-type background. This method is proving to be a powerful tool in oncology and immunology in diagnostics and research.

Do you have any questions about ddPCR? Are you interested in hiring a ddPCR service provider for your project? Contact MOgene to find out how we can help you in this process.