Flow cytometric analysis of CD83 expression on cultured human dendritic cells. Human peripheral blood monocytes were treated with 20 ng/ml of Recombinant Human IL-4 (Cat. No. 554605), and 20 ng/ml Recombinant Human GM-CSF (Cat. No. 550068) proteins for 7 days at 37°C. In addition, 20 ng/ml Recombinant Human TNF (Cat. No. 554618) was added for the last two days of culture. The cells were then stained with either PE-Cy™5 Mouse IgG1, κ Isotype Control (Cat. No. 555750; dashed line histogram) or PE-Cy™5 Mouse Anti-Human CD83 antibody (Cat. No. 551058; solid line histogram). The fluorescence histogram showing CD83 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable dendritic cells. 金山科研平台是一家专注细胞生物学试剂开发、销售、服务的高新技术企业。 1.进口胎牛血清：美国GIBCO、德国PAN-biotech、以色列BI、美国GEMINI、美国Hyclone等。 2.OriGen细胞冻存产品，TAKARA、Biolog微生物鉴定板、梅里埃试剂、Borer清洗液、台湾旭基产品。 3.Thermo、SIGMA、QIAGEN、MERCK、LEICA、Millipore、Whatman、MP Bio、日本同仁等。 好产品+好价格+好服务为经营理念，希望不断发展的金山科研平台成为您 的选择！