The EasySep™ Mouse Plasmacytoid DC Isolation Kit is designed to isolate plasmacytoid dendritic cells (pDCs) from single-cell suspensions of splenocytes or other tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies that are directed against non-pDCs. Labeled cells are then recognized by Tetrameric Antibody Complexes that are directed against biotin and dextran. These cells are bound to magnetic particles and separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.