Brand:BD Pharmingen™
Alternative Name:MAC-1A; Mac-1; ITGAM; Integrin al pha M; CR3A; CR-3 alpha; Mo1; SLEB6
Reactivity: 人类(QC测试),恒河猴,犬尾藻,狒狒(在开发中测试)
Isotype:Mouse IgG1, κ
Immunogen:Human monocytes
Application: 流式细胞术(常规测试)
Vol. Per Test:20 μl
Workshop Number:IV M047
Entrez Gene ID:3684
RRID:AB_395789
Storage Buffer:Aqueous buffered solution containing BSA and ≤0.09% so dium azide.
Regulatory Status:RUO
准备和存储
在4°C下未稀释储存,并防止长时间暴露在光线下。不要冷冻。将单克隆抗体通过亲和色谱从组织培养物上清液或腹水中纯化。在 条件下将抗体与R-PE偶联,并去除未偶联抗体和游离PE。
推荐的实验流程
BD™CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application.
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