® Human Platelet Lysate (Research Grade)

Safety Information & Precautions Products not intended for direct use in animals or humans. All PLTGold® donors have been tested for infectious diseases; however, as a blood derived product, PLTGold® should be handled and treated as potentially infectious. Universal precautions for handling and disposal of biological products should be used when working with PLTGold® . Using PLTGold® Thaw at 37°C or 4°C. It is not recommended to expose PLTGold® to repeated temperature changes that could affect the integrity of its components. For that reason, we recommend thawing the product and preparing aliquots as soon as it is received. Aliquots can be stored at -20°C or colder protected from light. Storage at 4°C is recommended for periods no longer than 2 weeks. Complete media can be prepared, aliquoted and stored at -80oC for up to 9 months. Do not store complete media at 4oC for longer than 2 weeks. Filtration of PLTGold® by itself is not recommended. Filtration of complete media containing PLTGold® may be performed, if necessary. Culture Conditions Using PLTGold® Cell seeding should be performed following the general guidelines for the specific cell type. For Mesenchymal Stem Cells (MSCs), cells are typically plated at approximately 2x103 – 5x103 cells per cm2 . For MSCs, PLTGold® can be used at 5% vol/vol in a typical cell culture medium such as DMEM or α-MEM. If the basic media doesn’t contain Glutamine, a source of L-Glutamine will need to be added to the media at a final concentration of 2mM. For other types of cells, the concentration of PLTGold® will need to be titrated according to the application (a titration from 2% vol/vol to 10% vol/vol is recommended to establish the percentage of PLTGold® needed for the cell type to use). Document: 0094-rev08 Do not allow primary MSC confluence to exceed 70-80%. Origin PLTGold® was developed to eliminate the need for heparin in hPL. It is an unfractionated product derived from our original hPL, PLTMax® that does not contain or require the addition of heparin. PLTGold® remains clot free and with similar performance to PLTMax® . References Crespo-Diaz R, Behfar A, Butler GW, et al. Platelet lysate consisting of a natural repair proteome supports human mesenchymal stem cell proliferation and chromosomal stability. Cell Transplant. 2011;20(6):797-811. Burnouf T, Strunk D, Koh MB, et al. Human platelet lysate: Replacing fetal bovine serum as a gold standard for human cell propagation Biomaterials. 2016 Jan;76:371-87. Alonso-Camino V, Clarke B, Nielsen J, et al. In vitro expansion of mesenchymal stem cells using media supplemented with unfractionated heparin-free platelet lysate. Poster presented at: ISCT Annual Meeting. London, UK. 2017 May. Bulur P, Wiltshire T, Dudakovic A, et al. Impact of media supplementation on the secretion of IFN-γ induced indoleamine 2-3 deoxygenase and resultant immune suppression by mesenchymal stromal cells. Poster presented at: ISCT Annual Meeting. Montreal, Canada. 2018 May. Alonso-Camino V, Mirsch W. In vitro expansion of human primary endothelial cells for clinical use using EndoGo™ XF Medium supplemented with PLTGold® human platelet lysate. Poster presented at: ISCT Annual Meeting. Montreal, Canada. 201